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STEMCELL Technologies Inc easysep human myeloid dc enrichment kit
Easysep Human Myeloid Dc Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+human+myeloid+dc+enrichment+kit/easysep+human+myeloid+dc+enrichment+kit/pm40562926-630-19-25
Average 90 stars, based on 1 article reviews
easysep human myeloid dc enrichment kit - by Bioz Stars, 2026-11
90/100 stars

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Isolation:

Article Title: Chlamydia trachomatis impairs T cell priming by inducing dendritic cell death.
Article Snippet: .. Mononuclear cells were isolated using Lympholyte-H Cell Separation Media (Cedarlane), and dendritic cells were purified using an EasySep Human Myeloid DC Enrichment Kit (StemCell Technologies) following the manufacturer’s instructions. .. Cells were then infected with C. trachomatis and cultured in RPMI (Invitrogen) supplemented with 10% FBS, L-glutamine, HEPES, 50 mM 2-mercap toethanol, and 500 IU/mL human GMCSF (Peprotech).

Article Title: The Th1/Tfh-like biased responses elicited by the rASP-1 innate adjuvant are dependent on TRIF and Type I IFN receptor pathways.
Article Snippet: .. Isolation of untouched cDCs was performed using immunomagnetic negative selection using a cocktail of antibodies that bind to cells that are negative of myeloid DCs (EasySep human myeloid DC enrichment kit, StemCell Technologies). ..

Article Title: Antigen binding molecules targeting thymic stromal lymphopoietin (TSLP)
Article Snippet: Myeloid Dendritic Cell (mDC) TSLP Stimulation Assay Peripheral Blood Mononuclear Cells (PBMCs) were isolated from fresh whole blood by Ficoll-Paque density centrifugation from 5 independent donors and cryopreserved in medium containing 90% fetal bovine serum and 10% DMSO. .. PBMCs were thawed into PBS containing 5 mM EDTA and 2% FBS, counted and myeloid dendritic cells (mDCs) were isolated using StemCell Technologies EasySep Human Myeloid DC Enrichment Kit. .. In a 96-well maxi-sorp (Thermo Fisher Scientific) plate serial dilutions of anti-TSLP antibodies in 25 μl of Assay Media (RPMI-1640+10% FBS) are pre-incubated with 25 μl of 1 ng/ml hTSLP cytokine (BioLegend) at 37° C. for 30 minutes.

Purification:

Article Title: Chlamydia trachomatis impairs T cell priming by inducing dendritic cell death.
Article Snippet: .. Mononuclear cells were isolated using Lympholyte-H Cell Separation Media (Cedarlane), and dendritic cells were purified using an EasySep Human Myeloid DC Enrichment Kit (StemCell Technologies) following the manufacturer’s instructions. .. Cells were then infected with C. trachomatis and cultured in RPMI (Invitrogen) supplemented with 10% FBS, L-glutamine, HEPES, 50 mM 2-mercap toethanol, and 500 IU/mL human GMCSF (Peprotech).

Article Title: B7-H4 reduction induced by Toxoplasma gondii infection results in dysfunction of decidual dendritic cells by regulating the JAK2/STAT3 pathway.
Article Snippet: .. Purification of dDCs from monocytes was performed using the EasySep Human Myeloid DC Enrichment Kit (STEMCELL Technologies, Canada) according to the manufacturer’s instructions, with > 95% purity ensured for experiments. ..

Article Title: Editor's page: Focus Issue Coronary Artery Disease.
Article Snippet: Received 12 December 2018; editorial decision 12 August 2019; accepted 20 August 2019; published online October 23, 2019.. This article is dedicated to the memory of our beloved friend and amazing colleague, Ryota Funakoshi, who recently passed away.. He is deeply missed by all of us.

Article Title: B7-H4 reduction induced by Toxoplasma gondii infection results in dysfunction of decidual dendritic cells by regulating the JAK2/STAT3 pathway
Article Snippet: .. Purification of dDCs from monocytes was performed using the EasySep Human Myeloid DC Enrichment Kit (STEMCELL Technologies, Canada) according to the manufacturer’s instructions, with > 95% purity ensured for experiments. ..

Selection:

Article Title: The Th1/Tfh-like biased responses elicited by the rASP-1 innate adjuvant are dependent on TRIF and Type I IFN receptor pathways.
Article Snippet: .. Isolation of untouched cDCs was performed using immunomagnetic negative selection using a cocktail of antibodies that bind to cells that are negative of myeloid DCs (EasySep human myeloid DC enrichment kit, StemCell Technologies). ..

other:

Article Title: Rescuing dendritic cell interstitial motility sustains antitumour immunity.
Article Snippet: The dendritic cell (DC)-initiated and sustained cancer immunity cycle is indispensable for effective endogenous and therapeutically mobilized antitumour T cell responses.. This necessitates the continuous migration of antigen-carrying DCs from the tumour microenvironment (TME) to the tumour draining lymph nodes (tdLNs).. Here, through longitudinal analysis of human and mouse tumours, we observed a progressive decrease in migratory conventional DCs (mig-cDCs) in the tdLNs during tumour progression.

Article Title: High-parametric evaluation of human invariant natural killer T cells to delineate heterogeneity in allo- and autoimmunity
Article Snippet: T cells were labeled with CellTrace Violet (Thermo Fisher Scientific) according to manufacturer’s instructions.



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